Product Details
Key Features of Pyrochrome Chromogenic LAL Reagent
- Maximum Sensitivity: Pyrochrome achieves a maximum sensitivity of 0.001 EU/mL, making it one of the most sensitive chromogenic LAL reagents on the market. This level of sensitivity is crucial for detecting low levels of endotoxins in highly diluted samples or in products with stringent endotoxin limits.
- Flexible Testing Ratios: Pyrochrome can be used with the Pyros Kinetix® Flex tube reader at either a 1:1 or an economical 4:1 sample-to-lysate ratio, allowing for cost-effective testing without compromising sensitivity.
- Adaptable for Various Assay Formats: This chromogenic reagent is suitable for both kinetic and endpoint assays. The diazo method option is available for testing samples with color interference, shifting the absorption spectrum and improving test accuracy.
- Highly Cost Effective: Pyrochrome is designed to be a cost-effective solution for endotoxin testing, offering high sensitivity and reliable performance at an economical price point.
Assay Sensitivity and Performance
The sensitivity of chromogenic assays is determined by the lowest standard concentration on the standard curve used for the assay. The maximum sensitivity of Pyrochrome is 0.001 EU/mL when run in a Pyros Kinetix Flex tube reader or incubated microplate reader with Glucashield Buffer.
Sample-to-Lysate Ratios
In the Pyros Kinetix Flex tube reader, Pyrochrome can be used at an economical ratio of 4:1 using 50 µL of reagent per well or 1:1 using 100 µL/well. When used in a microplate reader, the reagent is used at a ratio of 1:1 and a volume of 50 µL/well (60 tests/vial) or 100 µL/well (30 tests/vial).
Test Performance
For optimal test performance, the Pyrochrome/sample mixture should be incubated at 37±1°C and read using a microplate reader equipped with a 405–410 nm filter or 540–550 nm filter for the diazo method. The incubation time depends on the lowest standard concentration in the standard curve. Software is used to construct the standard curve and calculate the endotoxin concentrations.
Product Stability
Once reconstituted, Pyrochrome is stable for 8 hours when stored at 2–8ºC.
Reconstitution
Pyrochrome lysate is reconstituted with an optimized Pyrochome reconstitution buffer (C1500). Pyrochrome can also be reconstituted with Glucashield buffer, a (1→3)- β-D-glucan inhibiting buffer, to render the assay endotoxin specific (CG1500).
Ordering Information
Pyrochrome is offered with a choice of reconstitution buffer and is recommended for use with the 10 ng/vial Control Standard Endotoxin (CSE, EC010). Certificates of Analysis, specific to the Pyrochrome and CSE lot, can be obtained from ACC or online at www.acciusa.com.
Safety Data Sheets
Click on the links below to view Safety Data Sheets.
United States
Pyrochrome® Reconstitution Buffer
United Kingdom
Pyrochrome® (BE)
Pyrochrome® Reconstitution Buffer (BE)
Europe
Pyrochrome® (DE)
Pyrochrome® (ES)
Pyrochrome® (FR)
Pyrochrome® (IT)
Pyrochrome® (PT)
Pyrochrome® Reconstitution Buffer (DE)
Pyrochrome® Reconstitution Buffer (ES)
Pyrochrome® Reconstitution Buffer (FR)
Pyrochrome® Reconstitution Buffer (IT)
Pyrochrome® Reconstitution Buffer (PT)
Canada
Pyrochrome® (EN)
Pyrochrome® (FR)
Pyrochrome® Reconstitution Buffer (EN)
Pyrochrome® Reconstitution Buffer (FR)
Mexico
Product Literature
Click on the links below to view product literature.
Product Codes
| Product Description | Product Code |
|---|---|
| 5 Pack (300 Test) | #C1500-5 |
| 25 Pack (1500 Test) | #C1500-25 |
| 5 Pack (300 Test with Glucashield Buffer) | #CG1500-5 |
| 25 Pack (1500 Test with Glucashield Buffer) | #CG1500-25 |
Partner with the Experts
Ready to optimize your endotoxin testing with the most reliable chromogenic LAL reagent? Get expert guidance from the specialists at ACC. Whether you need help selecting the right product or have questions about test methods, our experienced team is here to support you every step of the way.
Contact us today to ensure your testing is accurate, compliant, and efficient.
